Plant Tissue Cult. 7(2) 97-102, 1997 (December)

Selection and Regeneration of Hydroxy Proline resistant Cell lines of Finger Millet (Eleusina coracanai L.)

R. V. Ramana, Ch. Venu, T. Jaya Sree and A. Sadanadam

Department of Botany, Kakatiya University, Warangal-506 009, A.P., India

Key words: Regeneration, finger millet, Amino acid analogue, Somaclonal variation

Abastract

The callus from mature caryopsis of two cultivars of finger millet proliferated on MS medium supplemented with 2,4-D and 0.5, 1,2,3,4, 5mM of hydroxy proline (hyp). The growth rate at mM was normal. Increased levels, from to 2-5 mM lead to a decrease in the growth and beyond 5 mM, drastic inhibition of callus growth was observed. Plants regenerated from the hyp tolerant callus.

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