Plant Tissue Cult. 7(1) : 35-39, 1997 (June)

A Simple Protocol for Efficient Plantlet Regeneration from Tomato (Lycopersicon esculentum Mill.) Hypocotyl Derived Callus

M. Jawahar, S. Varisai Mohamed and N. Jayabalan

Mutation Breeding and Tissue Culture Unit, Department of Plant Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli- 620 024, Tamil Nadu, India

Key words: Lycopersicon esculentum, Hypocotyl, Callus, Plant regeneration

Abastract

Callus was induced from hypocotyl explants of tomato (Lycopersicon esculentum Mill.) on MS medium supplemented with IAA 2 mg/1 and BAP 1 mg/1 and maintained on the same medium. The plantlets were rooted on MS medium with IAA 2.5 mg/1 and were successfully hardened and transferred to the field.

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