Plant Tissue Cult. & Biotech. 35(2): 229-236, 2025 (December)

General

Micropropagation of Withania somnifera Using Shoot Tip Explants

Youjanya G. Dongardive, Vijayalakshmi S. Salunkhe and Rupali R. Taur

Department of plant Biotechnology, Institute of Biosciences and Technology, MGM University, Chh. Sambhajinagar, Pin code-431003, Maharashtra, India

Key words: Withania Somnifera, Micropropagation, In vitro culture, Shoot tip

Abastract

In the present investigation in vitro propagation of a rare medicinal plant, Withania somnifera was carried out. Direct regeneration achieved from shoot tip explants and shoot multiplication was obtained by using cytokinin BAP (2.0 μM /l) and combination of BAP (2.0 μM /l) + IBA (2.0 μM/l), respectively. MS media with shoot tip explants supplemented with BAP (2.0 μM/l) produced maximum average number of shoots (5 ± 0.53) and average shoot length was found to b 2.5 ± 0.15 cm. significant induced were sub cultured in shoot multiplication, developed shoot multiplication was obtained average number of shoots (5.3 ± 0.41) and average shoot length 6.5 ± 0.12 cm was shown on MS media in combination with BAP (4.0 μM) + IBA (2.0-8.0 μM/l). Maximum average number (6 ± 0.10) and average length 7.6 ± 0.18 cm of roots were observed on MS media supplemented with IBA (2.0 um/l). The MS medium supplemented with BAP and Kn was formed to be suitable for in vitro propagation of W. somnifera.

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