Plant Tissue cult. 3(1) : 41-45, 1993

In Vitro Multiple Shoot Regeneration from Bulb Scale and Leaf Segments of Pancratium biflorum Roxb.

Selima Begum and Syed Hadiuzzaman

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Pancratium biflorum, Bulblet, Multiple shoot

Abastract

Regeneration of multiple shoots via bulblet induction from aseptically cultured bulb scale and leaf segments of Pancratium biflorum Roxb. Was accomplished by the addition of different combinations of NAA and BAP to the MS, modified MS (MMS) and B5 media. A large number of bulblets was induced when bulb scale segments were cultured in MMS medium containing 1.0 mg/1 NAA + 4.0 mg/1 BAP. The induced bulblets produced shoots when transferred to MS medium supplemented with 0.5 mg/1 NAA + 3.0 mg/1 BAP. On the other hand, leaf segments produced a large number of adventitious bulblets on B5 medium supplemented with 1.0 mg/1 NAA + 0.2 mg/1 BAP. On transfer to MS medium containing 1.0 mg/1 NAA + 4.0 mg/1 BAP, the bulblets produced shoots. Healthy roots in both the cases were produced when individual shoots were cultured on MS medium containing 0.5 mg/1 IBA.

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