Plant Tissue Cult. & Biotech. 35(1): 177-184, 2025 (June)

General

Establishment of Direct Shoot Regeneration in Spilanthes acmella Murr. Using Leaf Explants - An In vitro Approach for Mass Propagation

Hajera Sana* and A. Sabitha Rani¹

Department of Botany, Veeranari Chakali Ilamma University, Hyderabad-500095, Telangana State, India

Key words: Key words: Direct regeneration, Leaf explants, Spilanthes acmella, In vitro propagation, Multiple shoots

Abastract

Direct shoot regeneration was obtained from leaf explants of Spilanthes acmella Murr. on MS medium supplemented with different combinations of plant growth regulators like BAP and in combination with NAA and IAA. Among all, 2.0 mg/l BAP produced 70% of shoot regeneration from leaf explants. The BAP at 2 mg/l BAP and 1 mg/l NAA gave the highest percentage of response (90%). The best treatment was observed at 2.0 mg/l BAP + 1.0 mg/l IAA with 80% of regeneration. The multiple shoots were sub cultured on the same media for further multiplication of shoots. Among the different combinations, 2 mg/l BAP + 1 mg/l NAA induced the highest number of multiple shoots i.e. 17 shoots in first subculture and 35 shoots in second subculture respectively. The BAP at 2 mg/l + 1 mg/l IAA induced the highest number of shoots i.e. 18 shoots in first subculture and 40 shoots in second subculture. Regenerated shoots developed efficent root system in half strength of MS medium with different concentrations of IBA and NAA. Among all, 1.0 mg/l IBA induced highest rooting percentage (90%) and 1.0 mg/l NAA induced 85% rooting. In vitro regenerated plantlets were established well in the field with 95% survival rate. This study appeared to be very useful for direct regeneration and mass propagation through in vitro culture of leaf explants.

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