Plant Tissue Cult. & Biotech. 34(2): 165-171, 2024 (December)

General

Development of Anther Culture Protocol for Selected Brinjal Germplasm (Solanum melongena L.)

P.M.L. Pathmathilaka, H.M.P.S. Kumari and P.A. Weerasinghe

Department of Plant Sciences, Faculty of Agriculture, Rajarata University of Sri Lanka, Anuradhapura, Sri Lanka

Key words: Anther Culture, Brinjal (Solanum melongena L.), Callus induction, Double haploid, Plant growth regulators

Abastract

Selected anthers from seven brinjal germplasm namely; Anjalee, Padagoda, EGH-5, EGH13, EGH-16, V-5, and 8104 were cultured on MS medium containing different hormonal supplements for callus induction and in vitro regeneration. Various hormonal treatments (T1 to T4) for callus induction consisted 1.0 mg/l Kn and 2.0 mg/l BAP with different concentrations (1, 3, 5 and 0 mg/l) of NAA. The highest percentage of callus induction (83.33 ± 16.67, p < 0.05) was obtained in T2 from the hybrid germplasm EGH-13. Significantly the highest percentage of root formation (83.33 ± 16.67, p < 0.05) was obtained on 1 mg/l BAP from EGH-13. MS supplemented with 1 mg/l BAP was found to produce shoot (6.67 ± 6.67, (p <0.05) only for germplasm Padagoda. All other germplasm failed to produced shoots on any medium having different combination of hormonal supplements.

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