Plant Tissue Cult. & Biotech. 34(1): 1-10, 2024 (June)

General

Regeneration of a Multipurpose Woody Legume - Acacia auriculiformis through Mature Tree Nodal Explants

Shruti Banswal^*, Mudita Prakash¹, P. Jyotirmayi² and Shrish C. Gupta³

Shaheed Rajguru College of Applied Sciences for Women, University of Delhi, Vasundhara Enclave, Delhi 110096, India

Key words: Mature tree nodes, Acacia auriculiformis, In vitro, Regeneration

Abastract

Multiple shoots have been induced in nodal explants of 30-year-old Acacia auriculiformis trees on Nitsch's medium (N medium) supplemented with 1.0 mg/l N6-benzyladenine. Addition of 0.1 mg/l adenine sulfate to N medium containing BA, enhanced the caulogenic response whereby the number of shoots developing on each node increased from three to fifteen. Plantlets were obtained by rooting axenically raised shoots on N medium (half strength) with 1.0 mg/l naphthalene acetic acid. Use of activated charcoal (0.01%) in the medium completely suppressed callus formation at the root-shoot junction.

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