Plant Tissue Cult. 6(2) : 127-134, 1996

Plantlet Regeneration from Callus in Picrorhiza kurroa Royle ex Benth. ? An Endangered Medicinal Plant

Nanda Lal¹ and paramvir Singh Ahuja²

Division of plant Tissue Cutture, Central Institute of Medicinal and Aromatic Plant (CIMAP), Lucknow 226 015, India

Key words: Plant regeneration, Callus, Picrorhiza kurroa, Endangered plant

Abastract

MS medium supplemented with 0.5-2.0 mg/1 2,4-dichlorophenoxyacetic acid (2,4-D) induced callus from cuttings and leaf explants of Picrorhiza kurroa Royle ex Benth. within two weeks. Maintenance and proliferation of callus were optimal on MS medium containing 4.0 mg/1 a-naphthaleneacetic acid (NAA) and 1.0 mg/1 kinetin (Kn). Shoot organogenesis wasd achieved from callus by its sequential transfer at different media i.e. induction of bud primordial on MS medium with 0.25 mg/1 6-benzylaminopurine (BAP) and conversion of bud primordia into shoots on MS + 0.12 mg/1 BAP (with half strength nitrogen supply). Single combinations of media did not favour shoot bud/somatic embryo formation in this species. Regenerated shoots were rooted on MS medium containing 0.2 mg/1 NAA. Four-week-old plantlets were successfully established in soil.

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