Plant Tissue Cult. & Biotech. 32(2): 227-236, 2022 (December)

General

In vitro Micropropagation of Berberis chitria (Lindl.) - A Rare Medicinal Plant from Himachal Pradesh, India

Shagun Sharma, Harcharan S. Dhaliwal¹ and Vivek Sharma^*

Department of Botany, Akal College of Basic Sciences, Eternal University, Baru Sahib-173101 (H.P.) India

Key words: Berberis chitria (Lindl.), In vitro micropropagation, Callus induction, Shoot induction, Root induction.

Abastract

An efficient in-vitro micropropagation protocol has been developed for Berberis chitria (Lindl.) - a rare medicinal plant from Himachal Pradesh, India using seeds and in-vitro nodal explants. The effects of different concentrations of BAP and GA3 for in-vitro seed germination was investigated and consequently 6.0 mg/l BAP + 0.0 mg/l GA3 was reported to be excellent for seed germination. Callus induction from leaf explants from aseptically grown seedling were also studied by using various concentrations of BAP and 2, 4-D and showing 94.26

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