Plant Tissue Cult. & Biotech. 32(2): 217-226, 2022 (December)

General

Development of In vitro Mass Propagation Protocol for Gerbera (Gerbera jamesonii Bolus) var. Orange

Nasrin Akter, Selim Sarkar, Naimul Hasan, Shaikh Mizanur Rahman and Md. Abdur Rauf Sarkar^*

Department of Genetic Engineering and Biotechnology, Jashore University of Science and Technology, Jashore 7408, Bangladesh

Key words: Mass propagation, Gerbera, Flower bud, Hormone-free rooting, Acclimatization

Abastract

An in vitro mass propagation protocol was developed for Gerbera (Gerbera jamesonii Bolus) var. orange using flower bud explants. The young (7 days) explants were found to be suitable for culture and highest (100%) callus induction was obtained in MS medium supplemented with 5.0 mg/l BAP with 1.0 mg/l NAA. The maximum shoot regeneration (95%) and the number of shoots (10) were observed in MS medium supplemented with 6.0 mg/l BAP with 0.3 mg/l NAA. The highest shoot multiplication frequency (95%) and the number of shoots (60) were obtained in MS medium supplemented with only 2.0 mg/l BAP. The well-developed highest number of roots (5.0) per plant and average root length (5.0 cm) with 100% root induction efficiency without callusing was recorded in the half strength of MS medium devoid of any hormonal supplement. Acclimatized in vitro grown plantlets were successfully transferred into the field condition.

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