Plant Tissue Cult. & Biotech. 32(2): 193-203, 2022 (December)

General

In vitro Flowering and Production of Seeds in Oxalis corniculata L. - an Important Medicinal Plant

V.N. Swetha Prasuna, C.M. Narendra Reddy, M. Rajagopal, G. Sudhasri Purna and B. Srinivas^*

Department of Biotechnology, School of Herbal Studies and Naturo Sciences, Dravidian University, Kuppam-517425, Andhra Pradesh, India.

Key words: Plant growth regulators, In vitro flowering, Oxalis corniculata, production of seeds, medicinal plant.

Abastract

The multiple shoots (14.2) from Oxalis corniculata differentiated through bud breaking on MS medium with 2.0 mg/l BAP. The regenerated shoots were further sub-cultured on MS medium containing 1.0 mg/l BAP and 0.5 mg/l NAA. Vegetative to reproductive phase transition occurred in the cultures affected by plant growth regulators (PGRs), sucrose concentrations, strength of MS salts and light. Flower buds initiated from the regenerated shoots on half strength of MS salts with 1.0 mg/l Kn + 0.5 mg/l NAA and 3% sucrose. These flower buds opened under low light (10-15

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