Plant Tissue Cult. & Biotech. 32(1): 13-20, 2022 (June)

General

Micropropagation of Alocasia amazonica through Indirect Shoot Organogenesis

Raihan Iqbal Raju^*, Asma Kanon Hashi, Ahshan Jazib and Mohd. Talim Hossain

Department of Botany, Jahangirnagar University, Savar, Dhaka-1342, Bangladesh

Key words: Micropropagation, Alocasia amazonica, Indirect shoot organogenesis.

Abastract

The present experiment was conducted to establish an efficient in vitro regeneration protocol for Alocasia amazonica through indirect organogenesis from young leaf segment. For callus induction, leaf explants were inoculated on MS medium containing different concentrations of 2,4-D. Highest callus induction frequency (90%) was recorded on MS medium supplemented with 4.0 mg/l 2,4-D. Remarkable results on indirect shoot organogenesis and multiplication (86.67%) with maximum shoot number per unit callus (11.64

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