Plant Tissue Cult. & Biotech. 31(2): 153-160, 2021 (December)

General

In vitro Propagation of Vanda testacea (Lindl.) Reichb. F, a Medicinally Important Threatened Orchid

Saranjeet Kaur^*

Faculty, University Institute of Biotechnology, Division Biosciences, Chandigarh University, District- Mohali, Punjab, India

Key words: Asymbiotic, Orthodox seeds, Protocorms, Orchids, Growth regulators

Abastract

The present study was planned to enable in vitro conservation of Vanda testacea, a highly medicinal orchid species through in vitro asymbiotic seed germination technique in Mitra orchid medium supplemented with cytokinins (Kn - 4.65 M, BAP - 4.44 M), and auxin (NAA- 5.37 M). The germination frequency and initiation of germination was higher in NAA augmented medium and seedlings developed in 12.50

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