Plant Tissue Cult. & Biotech. 31(2): 143-151, 2021 (December)

General

In vitro Regeneration of Picralima nitida (Stapf). T. Durand & H. using Zygotic Embryo

Kamdem, Nehemie Tchinda Donfagsiteli¹, Njouheretou Mfondi Mache, Carine Temegne Nono, Rodrigue Goimasse, Leila Zebaze Zambou¹, Justine Germo Nzweundji¹, Emmanuel Youmbi and Libert Brice Tonfack^*

Physiology and Plant Improvement Unit, Laboratory of Biotechnology and Environment, Department of Plant Biology, Faculty of Science, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon

Key words: 2,4-D, Acclimatisation, Disinfection, Picralima nitida regeneration, Zygotic embryogenesis.

Abastract

Disinfected mature seed embryos of Picralima nitida, were cultured in MS medium supplemented with different concentrations and combinations of 2,4-D, BAP and NAA to determine an efficient protocol for in vitro propagation. Nine culture media made of combination of different components were used in a factorial design with three replications. Results showed up to 80

Download Full Article