Plant Tissue Cult. & Biotech. 30(2): 243-252, 2020 (December)

Plant Regeneration from Protocorm Like Body (PLB) derived Callus of Dendrobium barbatulum Lindl.

Ashok N. Pyati^*

Plant Tissue Culture Laboratory, Department of Botany, Maharani

Key words: Protocorms, Dendrobium barbatulum, Callus culture

Abastract

In vitro regenerative potential of protocorm like bodies (PLBs) of Dendrobium barbatulum were assessed. The formation of secondary PLBs from the primary PLBs that formed from the callus, it is derived from a few larger injured protocorm like bodies. The injured larger PLBs were cultured on half strength MS supplemented with different concentrations of BAP (0.32, 1.62, 3.23 and 16.15

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