Plant Tissue Cult. & Biotech. 30(2): 219-229, 2020 (December)

High Efficiency In vitro Regeneration and Genetic Stability of Corallocarpus epigaeus - An Endangered Medicinal Plant

Spoorthi Veera, Pavani Chirumamilla¹ and Shasthree Taduri^*

Department of Biotechnology, Kakatiya University, Warangal -506009, TS, India

Key words: High Efficiency, In vitro regeneration, Multiple shoots, Genetic fidelity, ISSR

Abastract

Indirect regeneration of plantlets from multiple shoot induction of Corallocarpus epigaeus was obtained from leaf and nodal explants on MS with different concentrations of BAP in combination with IAA/IBA or IBA alone. Among all the combinations, BAP and IBA exhibited maximum regeneration. High frequency of multiple shoots (89%) was obtained on BAP (2.0 mg/l) and IBA (1.5 mg/l) in nodal explants. Maximum mean shoot length of 6.8

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