Plant Tissue Cult. 6(2) : 67-71, 1996

In vitro Organogenesis of a Tree Legume ? Albizia lebbeck L.

M. A. Reza, R. Islam, A. N. K. Mamun and O. I. Joarder

Department of Botany, Rajshahi University, Rajshahi-6205, Bangladesh

Key words: Plant regeneration, Cotyledon culture, Albizia lebbeck

Abastract

A procedure was developed which allows high frequency adventitious shoot regeneration from immature cotyledons of Albizia lebbeck. Prolific shoot regeneration occurred following an initial callus growth on MS medium containing 2.0 mg/1 BA and 0.2 ? 0.5 mg/1 NAA. Shoot elongation was best achieved when regenerating part of the explant was cut into small pieces and sub cultured on to elongation medium (MS + 0.1 mg/1 BA). Elongated shoots rooted on MS medium with 1.0 mg/1 IBA.

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