Plant Tissue Cult. & Biotech. 30(1): 57-63, 2020 (June)

In vitro Micropropagation of Bacopa monnieri (L.) Penn. - An Important Medicinal Plant

Md. Adnan Rahe, Sanjida Rahman Mollika, M. Salim Khan, Tanjina Akhtar Banu, G. M. Al Amin, Md. Ahsan Habib, Shahina Akter, Mousona Islam and Ripa Akter Sharmin

Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Key words: Regeneration, Bacopa monnieri, Leaf, Node

Abastract

Investigation on in vitro multiple shoot regeneration in Bacopa monnieri (L.) Penn. using leaf and nodal explants was carried out on MS containing various concentrations and combinations of BAP, Kn, NAA and 2,4-D. Of the two explants, leaf showed the best response towards shoot regeneration and subsequent plant development on MS with 1.0 mg/l BAP and 0.25 mg/l Kn. In this combination, the mean number of shoots/explant was 10.6

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