Planty Tissue Cult. 6(1) : 57-62, 1996

Callus Induction and Plant Regeneration from Different Explants of Solanum nigrum L. Seedlings.

Miskat Ara Akhter jahan and Syed Hadiuzzaman¹

Bangladesh Council of Scientific and Industrial Research (BCSIR), Mirpur Road, Dhanmondi, Dhaka-1205., Bangladesh

Key words: Callus induction. Plant regeneration, proliferation, Solanum nigrun seedling

Abastract

Callus induction and plant regeneration ability have been studied in Solanum nigrum L. from various explants namely, shoot tip, stem, leaf and root segments. Best regenerating callus induction was observed when the leaf segments were cultured on MS medium supplemented with 0.5 mg/1 NAA and 2.0 mg/1 BAP. Maximum numbers of shoots were obtained from leaf segment derived callus on MS medium containing 4.0 mg/1 BAP. Healthy roots were developed within 6-8 days when regenerated shoots were transferred to MS basal medium with 0.5 mg/1 IBA. Regenerated plantlets were successfully transferred to the field.

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