Plant Tissue Cult. & Biotech. 28(2): 215-222, 2018 (December)

Indirect in vitro Regeneration of Viola canescens Wall. ex, Roxb. by using Leaf Calli

Arun Kumar Khajuria^* and N.S. Bisht

Department of Botany, Plant Tissue Culture Laboratory, HNB Garhwal University Campus Pauri, Pauri (Garhwal)-246001, Uttarakhand, India

Key words: Viola canescens, Callus, Plant regeneration

Abastract

An efficient indirect plant regeneration protocol was developed for Viola canescens, an important medicinal herb used in broad spectra of diseases in number of folk medicines since aeon. Excessive use of this plant without any rehabilitating measure has led to decline its natural population. Present investigation reports the use of zeatin to regenerate the plant from the callus on MS following its acclimatization on the soil condition. Calli of the plant responded positively to zeatin and maximum number of shoots 13.07

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