Plant tissue Cult. 6(1) : 41-49, 1996

In vitro Clonal Propagation of Gliricidea sepium Through Tissue Culture

C. Mukhopadhyay, S. C. Santra and P. D. Ghosh¹

Department of Ecological Studies, School of Environmental Science, University of Kalyani, Kalyani-741 235, West Bengal, India

Key words: Differentiation, Organogenesis, Regeneration, Multiple shoots

Abastract

Multiple shoots were obtained from nodal segments of young and mature tree of Gliricidea sepium on MS medium (Murashige and Skoog 1962) supplemented with 2, 4-D (0.5 to 2 mg/1) and Kn (0.5 to 1 mg/1). Rooting was achieved on transfer of the excised shoots to MS in combination with 2, 4-D (0.5 mg/1) and Kn (0.1 mg/1). At higher concentration of Kn and 2, 4-D callus tissues were unable to differentiate. The plantlets, which originated after 15 days were assicoated with roots. Histological analysis of organogenetic development has been investigated.

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