Plant Tissue Cult. & Biotech. 28(1): 141-146, 2018 (June)

Short Communications

Effects of Ethrel on Organogenesis of Protocorm-like bodies in Dendrobium kingianum In vitro

Sultana Umma Habiba^* , Md Monjurul Ahasan¹ and Kazuhiko Shimasaki¹

Faculty of Agriculture, Kochi University, B200 Monobe, Nankoku, Kochi 783-8520, Japan.

Key words: Dendrobium, Organogenesis, 6-benzylaminopurine , Protocorm-like body, Ethrel.

Abastract

This study attempted to understand the effects of BAP and ethrel on organogenesis of protocorm-like bodies (PLBs) in Dendrobium kingianum under white fluorescent lamps in vitro. BAP is the most effective cytokinin for multiplication in plant tissue culture. Ethrel regulates many aspects of plant morphogenesis. The highest number of PLBs (13.1) as against in control (8.1) and the highest number of developing shoots (2.1) was recorded in the medium containing 0.1 mg/l BAP combined with 1 mg/l ethrel. Increase in fresh weight showed higher values in same combination. Low concentration of BAP alone increased in the number of PLBs but showed inhibitory effects on shoot formation. On the other hand low concentration of ethrel alone increased both number of PLBs and shoots after 4 weeks in vitro.

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