Plant Tissue Cult. & Biotech. 27(2): 227-240, 2017 (December)

Effect of Methyl Jasmonate and Mannitol Application on Growth and Eugenol Content in Callus Cultures of Carnation

Mohamed A. Matter, Moemen S. Hanafy and Usama I. Aly*

Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Center, 33 El Behouth Street, Dokki, Giza, Egypt

Key words: Dianthus caryophyllus, Methyl Jasmonate, Mannitol, Eugenol, HPLC

Abastract

A protocol for callus induction from leaves of carnation is established. MS fortified with 2.0 mg /l 2,4-D and 0.5 mg/l Kn was found to be the appropriate medium for producing callus cultures. Methyl jasmonate at 20

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