Plant Tissue Cult. & Biotech. 27(2): 207-216, 2017 (December)

High Frequency In vitro Regeneration of Gynura procumbens (Lour.) Merr.

Tanjina Akhtar Banu, Barna Goswami, Shahina Akter, Mousona Islam, Tammana Tanjin, Ahashan Habib and Salim Khan*

Plant Tissue Culture Section, Biological Research Division, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh

Key words: Gynura procumbens, High frequency, Regeneration

Abastract

An efficient rapid in vitro regeneration protocol was described from nodal segment, leaf and petiole explants. MS medium supplemented with 1.0 mg/l BAP and 0.5 mg/l IAA was found best for the multiple shoot formation from nodal segments. In this combination 99% explants produced multiple shoots and the average number of shoots per explants was 20.1

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