Plant Tissue Cult. & Biotech. 27(2): 141-151, 2017 (December)

Aseptic Germination and Agrobacterium rhizogenes-mediated Transformation of Taraxacum hybernum Steven

Aleksey Knyazev*, Bulat Kuluev¹, Alexander Fateryga², Gulnar Yasybaeva¹ and Aleksey Chemeris¹

Institute of Biochemistry and Genetics, Ufa Scientific Centre, Russian Academy of Sciences, Ufa, Russia

Key words: Taraxacum hybernum, Taraxacum kok-saghyz, Russian Dandelion, Agrobacterium rhizogenes, Hairy Roots, Transgenic Plants, Natural Rubber

Abastract

Taraxacum hybernum Steven, also known as krym-saghyz, is one of the potential sources of natural rubber. The authors studied the aseptic germination of the T. hybernum seeds using various sterilizing agents and their effect on contamination. In vitro T. hybernum plants were used for Agrobacterium rhizogenes-mediated transformation. A4 and 15834 strains of A. rhizogenes showed similar effectiveness after inoculation by injection into the hypocotyls. Authors generated the hairy roots of T. hybernum and the transgenic forms of this plant using A. rhizogenes-mediated transformation.

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