Plant Tissue Cult. & Biotech. 26(2): 255-266, 2016 (December)

In vitro Regeneration of Blepharispermum subsessile DC: An Endangered Medicinal Plant of Odisha, India using Cotyledon Explants

Pranati Nayak and Kalidass C*

Taxonomy and Conservation Division, Regional Plant Resource Centre, Bhubaneswar-751015, Odisha, India

Key words: Blepharispermum subsessile, Endangered, In vitro regeneration, Cotyledon

Abastract

Multiple shoots were induced on cotyledon explants of in vitro grown seedlings of Blepharispermum subsessile DC, cultured on MS medium supplemented with various combinations and concentrations of BAP, IBA and GA₃. The highest regenerative response was observed on medium containing 2.5 mg/l BAP where shoot buds initiated after 12 days of inoculation and about 32 shoots were produced in 30 days time. Addition of GA₃ played a key role in leaf expansion and elongation of shoot buds. Addition of the auxin IBA to the induction medium resulted in more callus proliferation rather than shoot bud induction. The elongated shoots were transferred to root induction medium consisting of half strength MS supplemented with IAA, NAA and IBA. Highest rooting response (90%) was recorded in

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