Plant Tissue Cult. & Biotech. 26(2): 231-242, 2016 (December)

In vitro Salt Tolerance of Safflower (Carthamus tinctorius L.) Genotypes using Different Explants

Mahdi Hamedi, Pooran Golkar¹* and Ahmad Arzani

Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 8415683111, Iran

Key words: Cellular level, Salt tolerance, Carthamus tinctorius

Abastract

To evaluate the response of different genotypes of safflower (Carthamus tinctorius L.) to in vitro salt stress was conducted. Callus derived from leaflet, pedicel, hypocotyls, and adaxial and abaxial surfaces of the leaf were subjected to in vitro salt stress at 0, 100 and 200 mM of NaCl. The relative growth rate (RGR), callus growth rate (CGR), relative water content (RWC), tolerance index (TOL) and necrosis percentage were assessed. Results of analysis of variance indicated significant effects of salt stress, significant differences among genotypes for all traits and significant genotype

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