Plant Tissue Cult. & Biotech. 26(2): 187-195, 2016 (December)

In vitro Shoot Multiplication of Withania coagulans (Stocks) Dunal

Harsh Joshi, Sureshkumar Nekkala, Deepak Soner, Mafatlal M. Kher and M. Nataraj*

P.G. Department of Biosciences, Sardar Patel University, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Vallabh Vidyanagar-388120, India

Key words: Shoot multiplication, Withania coagulans, Hyperhydricity

Abastract

Withania coagulans (Stocks) Dunal is an important medicinal plant of Solanaceae. Nodal segments obtained from field grown plants were used as explants. 1 - 2 cm long nodal segment with a single one bud was cultured on MS containing 2.5 mg/l thidiazuron (TDZ), 0.1 mg/l NAA and 50 mg/l adenine sulphate (AdS) resulted in formation of 5.16 shoots per node. However, vitrification was observed in all explants within one month. On the other hand nodal explants cultured on MS supplemented with 2.50 mg/l meta-topoline (mT) with 0.1 mg/l NAA and 50 mg/l AdS resulted in the formation of 4.50 healthy and uniformly grown shoots per node.

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