Plant Tissue cult. 6(1) : 1-5, 1996

In vitro Differentiation of Plantlets from Tissue Cultures of Samanea saman

A.N.K. Mamun, R. Islam, M.A. Reza and O.I. Joarder

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Callus, Defferentiation, Plantlets, Raintree

Abastract

Hypocotyl explants of Samanea saman were excised from germinated seedlings and culture on MS basal medium supplemented with various combinations of cytokinins and auxins. Both cytokinin and auxin were required for induction of shoot organogenesis. Of the three cytokinins (in combination with a low concentration of NAA), BA was found to be the best for shoot regeneration. On this medium, hypocotyl explants invariably underwent callusing followed by multiple shoot formation. 2, 4-D in combination with any of the three cytokinins yielded a reduced number of shoot or none, but favoured good callus growth. When subcultured on media containing high concentration of BA or Kn and low concentration of NAA the latter also differentiated into shoots. The in vitro grown shoots, after transferring to MS + 1 mg/1 IBA developed roots. These plants have been successfully established in soils.

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