Plant Tissue Cult. & Biotech. 26(1): 131-141, 2016 (June)

Direct Organogenesis of Glinus lotoides L. - Anti-helmintic Herb Using Nodal Segments

Shiferaw Teshome¹* and Tileye Feyissa^1,2

Department of Biotechnology, Wolaita Sodo University, P.O. Box 138, Sodo, Ethiopia

Key words: Glinus lotoides; Kinetin, MS medium, Nodal segment

Abastract

Glinus lotoides L. is a valuable medicinal herb for the treatment of human and livestock ailments. However, it was listed under endangered plant species due to over utilization and lower seed viability. The purpose of this study has been to develop a micropropagation method from nodal segments of G. lotoides. MS containing various concentrations of Kn was used for culture induction. Best culture establishment (95%) and highest mean shoot number (2.45

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