Plant Tissue Cult. & Biotech. 25(1): 37-50, 2015 (June)

Effect of Silver Nitrate and Amino Acids on High Frequency Plants Regeneration in Barley (Hordeum vulgare L.)

Mozidul Haque, Abu Baker Siddique and S. M. Shahinul Islam*

Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Barley, Immature embryos, Somatic embryogenesis

Abastract

Three Bangladeshi barley genotypes viz. BARI barley-1, 3 and 6 were selected for this study to evaluate the efficiency of callus induction and plant regeneration. The effect of five doses of AgNO₃ singly, and combined with amino acids (L-proline, L-glutamine) on callus induction and plant regeneration efficiency was evaluated using BARI barley-3 and 6. The maximum values of callus induction were recorded at 49.20 and 32.66% for BARI barley-6 and 3, respectively when 2.0 mg/l AgNO₃ and 200 mg/l L-glutamine were added to the callus induction medium. Moreover, plant regeneration remarkably increased on MS + 1.0 mg/l BAP + 1.5 mg/l AgNO₃ + 150 mg/l L-glutamine as 37.20% in BARI barley-6 and 16.13% in BARI barley-3. For rooting AgNO₃ singly affect positively, whereas negative influence was observed in combinations with any amino acids. However, by using AgNO₃ and amino acids, around < 4, < 27 and < 5 fold increase in callus induction were obtained. Regeneration and rooting were also found to increase considerably.

Download Full Article