Plant Tissue Cult. & Biotech. 24(2): 263-271, 2014 (December)

Rapid in vitro shoot regeneration of Valeriana officinalis

Minoo Tansaz¹, Akram Zamani² and Mahmoud Otroshy*

Department of Plant Tissue Culture, Branch of Central Region of Iran, Agricultural Biotechnology Research Institute of Iran (ABRII), Isfahan, Najafabad, P. O. Box 85135-487, Iran

Key words: Valeriana officinalis, New combined formulations medium

Abastract

A protocol for efficient and rapid regeneration of medicinal plant (Valeriana officinalis L.) was developed by in vitro culture of crown division. The effect of three different culture media, MS, B5 and MB (a new combined medium) containing 12 combinations of BAP and NAA was evaluated on regeneration and growth parameters. Results revealed a significant response of media used for shoot regeneration. The best frequency of regenerated plantlets (80%) and number of regenerated shoots (9.43) were obtained on MB containing BAP (0.5 mg/l) + (0.25 mg/l). The maximum length of shoots was found in the MB with BAP (1 mg/l) + (0.5 mg/l). A positive influence of time was noticed on rooting of regenerated shoots. All acclimatized plantletswere successfully established in a greenhouse and showed normal growth. This protocol provides a technique for mass propagation of valerian and helpful for future genetic improvement studies.

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