Plant Tissue Cult. & Biotech. 24(2): 205-212, 2014 (December)

Assessment of Intraspecific Genetic Variation Between Three Morphotypes of Cissus quadrangularis using CCMP Markers

Ritesh Kaur and C. P. Malik*

School of Life Sciences, Jaipur National University, Jaipur, 302017, India

Key words: Consensus chloroplast microsatellite primers. Morphotypes. Cissus quadrangularis, Genetic relationship, Sequencing

Abastract

The objective of the current study was to assess and establish intraspecific variation between three morphotypes of Cissus quadrangularis using ten Consensus Chloroplast Microsatellite Primers. Significant level of genetic variation was detected between the three morphotypes. Sequence analysis of the CCMP PCR fragment showed the high degree of similarity to the chloroplast genome sequences of other Vitaceae members indicating that these regions are highly conserved.

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