Plant Tissue Cult. & Biotech. 24(1): 87-92, 2014 (June)

General

Induction of Direct Adventitious Shoot Regeneration in Pear (Pyrus communis L.)

Mahdieh Yousefiara, Maryam Jafarkhani Kermani, Abdolreza Bagheri¹, Ali Akbar Habashi and Hamid Abdollahi²

Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), Mahdasht Road, P.O. Box 31535-1897, Karaj, Iran

Key words: Induction, Direct shoot regeneration, Pyrus communis, Thidiazuron

Abastract

Efficient direct shoot regeneration of two pear (Pyrus communis L.), namely bartlett and dargazi was successfully developed for the use in future genetic engineering research. The basal MS and NN media supplemented with different concentrations of TDZ (0, 2.5, 5, 7.5 ?M) or BAP (0, 4, 8, 16 ?M) in combination with NAA (1 ?M) were compared. The result showed that direct adventitious shoot regeneration in pear was highly dependent on genotype, explant type and culture media.dargazi had higher rate of regeneration compared to bartlett and in both cultivars the highest per cent of regeneration was observed in lower sections of the leaves. Although the highest per cent of regeneration in bartlett(38) was attained in the NN medium containing 2.5 ?M TDZ and 1 ?M NAA, but the differences in shoot regeneration between media containing 5 or 7.5 ?M TDZ and 1 ?M NAA were not significant. The highest per cent of regeneration in dargazi (56) was obtained in NN medium containing 7.5 ?m TDZ and 1 ?m NAA.

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