Plant Tissue Cult. & Biotech. 24(1): 57-6463, 2014 (June)

Authentication of Silybum marianum Varieties Using RAPD Analysis

Sameh AbouZid

Pharmacognosy Department, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62111, Egypt

Key words: Silybum marianum, Authentication, RAPD analysis

Abastract

Silybum marianum is an important medicinal plant used for hepatic disorders. Two varieties exist for this plant S. marianum var. album (white-flowered) and S. marianum var. purple (purple-flowered). A fast and simple method (RAPD) was developed for authentication of S. marianum varieties (purple- and white- flowered) at a DNA level. The two varieties were distinguished by polymorphic bands generated by four decamer primers, namely, OPP-10, OPG-03, OPG-01, and OPC-17. The developed RAPD method will be useful for practical authentication of S. marianum varieties and their adulterants.

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