Plant Tissue Cult. & Biotech. 24(1): 37-45, 2014 (June)

An Improved System for Rapid in vitro Regeneration of Saintpaulia ionantha

Zahra Ghorbanzade* and Mohammad Ahmadabadi

Department of Biotechnology, Faculty of Agriculture, Azarbaijan Shahid Madani University, 35 km Tabriz-Maraqeh Road, Tabriz, Iran

Key words: Saintpaulia ionantha, Improved system, In vitro regeneration

Abastract

For rapid multiplication and genetic manipulation of African violets (Saintpaulia ionantha Wendl.) was aimed at developing a rapid and efficient regeneration and adaptation system from leaf explants. Using RM medium supplemented with different combinations of growth regulators, we developed a highly efficient and time-saving in vitro regeneration protocol. The developed system was also successfully applied for plant regeneration from petiole and internode explants of several Saintpaulia cultivars at a high efficiency. Regeneration occurred through both somatic embryogenesis and shoot organogenesis at a high frequency. Also, we developed a rapid root formation protocol followed by their efficient adaptation for in vitro regenerated plantlets.

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