Plant Tissue Cult. 5(2) : 119-124, 1995

In vitro Plantlets Differentiation in Kakrol (Momordica dioica Roxb.)

A. Hoque, R. Islam and O. I. Joarder

Department of Botany, University of Rajshahi, Rajshahi-6205, Bangladesh

Key words: Micropropagation, Adventitious shoots, ?Kakrol?, Momordica

Abastract

A procedure for adventitious shoot regeneration using hypocotyls explants of Momordica dioica has been developed. When MS medium was supplemented with 1.5 mg/1 BA and 0.1 NAA, 88% of hypocotyls explants regenerated shoots with an average number of 8.8 shoots per explant. The mean number of shoots was significantly reduced when sucrose concentration in the medium was less than 3%. Regenerated shoots were rooted on half-strength MS medium containing 1.0 mg/1 IBA. In vitro raised plantlets were transplanted to soil and are being evaluated for various agronomic traits.

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