Plant Tissue Cult. & Biotech. 24(1): 11-26, 2014 (June)

A Proteomic Analysis of Seed Proteins Expressed in a Brassica Somatic Hybrid and its two Parental Species

M. M. Hossain¹, X Li², I. H. Evans and M.A.Rahman

Department of Life Sciences, School of Science, University of Greenwich, Chatham Maritime, Medway, Kent ME4 4TB, U.K.

Key words: Brassica, Seed storage protein, LEA protein, Proteomics, Somatic hybrid, Vernalization

Abastract

Cabbage (Brassica oleracea) is one of the world?s major vegetable crops. However, the cabbage plant cannot be readily vernalized and produce seed in the tropics, whereas Chinese cabbage (Brassica campestris) can be conveniently vernalized at the seed stage. Proteins from seeds of an IPSA cabbage-Chinese cabbage somatic hybrid showing both hybrid vigour and the seed-stage vernalization characteristic were analyzed and compared with those of its two parents. 1D gel analysis indicated some differential expression of the seed proteins. Furthermore, in 2D, gel analysis, 13 differentially expressed protein spots were examined: of these ten were found to belong to the cruciferin seed storage protein complex, two were unidentifiable (no MASCOT match), and one was identified as late embryogenesis Abundant (LEA)-like protein, and was present only in the cabbage parent. This LEA-like protein in cabbage seed could therefore be implicated in interfering with the vernalization process in the tropics.

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