Plant Tissue Cult. & Biotech. 23(1): 000-00059-66, 2013 (June)

Evaluation of Genetic Relationship between Trigonella- Melilotus complex (L.) Using PCR- CCMP Markers

Amandeep Hora and C.P. Malik*

School of Life Sciences, Jaipur National University, Jaipur, Rajasthan, India

Key words: CCMP, DNA fingerprinting, Genetic correlation, Melilotus, Trigonella

Abastract

To distinguish the taxa at the specific and varietal levels a range of DNA fingerprinting techniques are being employed. The objective of the current study was to establish the genetic correlation between the Trigonella-Melilotus complex i.e. M. indica, M. alba and Trigonella polyceratia using ten Consensus Chloroplast Microsatellite Primers (CCMPs). The polymor-phism between the two genera indicated that they are intimately related and symbolize novel incongruity. Owing to less significant level of genetic variation, detected through CCMP, the differences between the two genera could be accredited to gene mutation or inconsequential chromosomal alterations.

Download Full Article