Plant Tissue Cult. 5(2) : 95-103, 1995

Androgenesis in Anther Cultures of an indica Rice cv. IR43

Himani Pande

Department of Botany, University of Delhi, Delhi 110 007, India

Key words: Androgenesis, indica rice, Heat shock, Colchicine, Green plants

Abastract

Effects of various parameters on the frequency of pollen embryogenesis in anther cultures of Oryza sativa ssp. indica cv. IR43 were studied. A combination of centrifugation (1000 rpm for 5 min) and cold treatment (10⁰C for 10 days) of the anthers enhanced androgenic response fivefold over the control. Heat shock (45⁰C for 5 min) and colchicine (50 mg/1) treatments also promoted androgenesis but were inferior to that of centrifugation. The frequency of green plants produced was distinctly higher in the first two treatments.

Download Full Article