Plant Tissue Cult. & Biotech. 23(1): 1-10, 2013 (June)

Application of Cobalt Chloride and Silver Nitrate for Efficient Microspore Culture of Brassica rapa ssp.

Khandakar Md. Rayhanul Kabir, Soon-Wook Kwon^1, and Yong-Jin Park^1*

Department of Plant Resources, College of Industrial Sciences, Kongju National University, Yesan 340-702, Republic of Korea

Key words: Microspore embryogenesis, Inhibitors of ethylene, Embryo yields

Abastract

The effects of ethylene antagonistic cobalt chloride and silver nitrate, on microspore embryogenesis were investigated using three different concentra-tions in the medium for three Korean cultivars (two non-heading and one heading) of Brassica rapa ssp. Inclusion of cobalt chloride (5 ?M ) in the culture medium significantly improved embryo production in the non-heading cultivar (33 embryos/bud) with embryo yields being increased up to 32%. The addition of silver nitrate (0.1 mg/l) to the culture medium also showed a progressive increase in embryo yields in the non-heading cultivar (34 embryos/bud) with embryo yields being increased up to 36%. For the heading cultivars, the highest embryogenic response was 2.8 embryos/bud (Jo saeng Miho), following the addition of silver nitrate (0.1 mg/l) to the culture medium, whereas 2.4 embryos/bud were observed with the addition of 5 ?M cobalt chloride to the culture medium.

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