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Plant Tissue Cult. & Biotech. 22(2): 137-142, 2012 (December)

Efficient in vitro Regeneration of a Medicinal Plant Harsinghar (Nyctanthes arbor-tristis L.)

S. Bansal, A.J. Bharati1 and Y.K. Bansal1*

Department of Botany, Hitkarini Mahila Mahavidyalaya Jonsganj, Jabalpur-482002 (M.P.), India

Key words: In vitro propagation, Nyctanthes arbor-tristis, Axillary bud, Multiple shoot

Abastract

In vitro propagation of Nyctanthes arbor-tristis L. has been successfully established from axillary bud explants on MS. Maximum number of multiple shoots was obtained on MS containing BAP (22.2 ?M). Half strength of MS (2% sucrose) supplemented with NAA (10.74 ?M) provided the maximum frequency of root initiation. The plantlets were successfully hardened.

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