Plant Tissue Cult. & Biotech. 21(2): 151-159, 2011 (December)

In vitro Propagation of Stemona tuberosa Lour. - A Rare Medicinal Plant through High Frequency Shoot Multiplication using Nodal Explants

Animesh Biswas*, M. A. Bari, Mohashweta Roy and S. K. Bhadra¹

Institute of Biological Sciences, Rajshahi University, Rajshahi-6205, Bangladesh

Key words: Nodal explant, Stemona tuberose, Rare medicinal plant, Direct organogenesis

Abastract

Nodal segments of Stemona tuberosa Lour. were found to proliferated without any differentiation on to MS supplemented with 3.0 mg/l BAP and 0.5 mg/l NAA under continuous dark condition. After 20 days of inoculation under dark condition the cultures were transferred to a daily cycle of 16/8 hrs light/dark photoperiod and there the proliferated nodal segments underwent direct organogenesis producing huge number of shoot buds (25.87/culture). The shoot buds underwent rapid elongation on a range of BAP (0.1 - 1.5 mg/l) and IBA (0.1 - 1.0 mg/l) supplemented MS. Rooting of elongated shoot buds was successfully achieved (90%) in half strength MS with 1.0 mg/l NAA. The plantlets were finally established in outside environment through a successive phase of acclima-tization.

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