Plant Tissue Cult. & Biotech. 21(1): 75-82, 2011 (June)

Rapid Micropropagation via Axillary Bud Proliferation of Coccinia grandis (L.) Voigt. from Nodal Segments

U. Thiripura Sundari, N. Ahmed Sherif, J.H. Franklin Benjamin and M. V. Rao*

Department of Plant Science, Bharathidasan University, Tiruchirappalli-620024, Tamil Nadu, India

Key words: Coccinia grandis, Axillary bud, Micropropagation, Nodal segments

Abastract

An efficient and rapid micropropagation protocol for Coccinia grandis (L.) Voigt. was successfully developed on MS fortified with Kn, BA and TDZ in various concentrations and in combination with IBA and IAA. Higher percentage (80) of response was obtained from nodal segments with an average number of 8.3 ? 0.9 shoots on MS + 0.5 mg/l Kn + 1.0 mg/l BA + 0.3 mg/l IBA. The isolated microshoots rooted (100%) on MS fortified with 0.1 mg/l IBA. The plantlets were hardened successfully with 80% survival rate. This efficient protocol will help the rapid multiplication of C. grandis for commercial propagation of this valuable medicinal plant.

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