Plant Tissue Cult. & Biotech. 20(1): 47-53, 2010 (June)

Efficient Plant Regeneration from Shoot Apices of Pearl Millet (Pennisetum americanum (L.) Leeke)

Y. Varalaxmi, A. Vijayalakshmi, K. Ravi Kumar, T. Vijayalakshmi and M. Maheswari*

Division of Crop Sciences, Central Research Institute for Dryland Agriculture,

Key words: Pearl millet, Efficient, Plant regeneration, Shoot apices

Abastract

An efficient and rapid regeneration protocol was developed from shoot tip explants of Pennisetum americanum (L.) Leeke variety WC-75. MS supplemented with low concentration of 2,4-D and Kn was most effective in producing embryogenic calli. Maximum regeneration potential of 40 shoots per calli were obtained when transferred to regeneration medium containing 1.0 mg/l Kn. Shoots developed were efficiently rooted within 15 days on the medium containing NAA. Over 90% of rooted plants were fertile after transfer to a net house.

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