Plant Tissue Cult. & Biotech. 20(1): 1-5, 2010 (June)

Histological Marker to Differentiate Organogenic Calli from Non Organogenic Calli of Gloriosa superba L.

Manju Madhavan* and Joy P. Joseph

Centre for Research and Post Graduate Studies, Department of Botany, Sacred Heart College, Thevara. Kochi 682013

Key words: Histological marker, Gloriosa superba, Organogenic calli

Abastract

A histological study of the indirect organogenesis from internodal cultures of Gloriosa superba L. showed that the callus initiated from the subepidermal cells. The organogenic and non organogenic callii are the result of hormonal variation in the medium. In non organogenic callus cells redifferentiated into xylem elements forming clusters of nest like structures. In organogenic callus, cells redifferen-tiated into nodules of meristemoids which further differentiated into shoot apical meristem.

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