Plant Tissue Cult. & Biotech. 19(2): 217-226, 2009 (December)

Sequence Specific Dihedral Angle Distribution: Application in Protein Structure Prediction and Evaluation

S. M. Minhaz Ud-Dean and Mahdi Muhammad Moosa*

Department of Genetic Engineering and Biotechnology, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Torsion angle, ?-? distribution, Sequence specific ramachandran plot, Ramasekharan, Protein structure appraisal

Abastract

Protein structure prediction and evaluation is one of the major fields of computational biology. Estimation of dihedral angle can provide information about the acceptability of both theoretically predicted and experimentally determined structures. Here we report on the sequence specific dihedral angle distribution of high resolution protein structures available in PDB and have developed Sasichandran, a tool for sequence specific dihedral angle prediction and structure evaluation. This tool will allow evaluation of a protein structure in pdb format from the sequence specific distribution of Ramachandran angles. Additionally, it will allow retrieval of the most probable Ramachandran angles for a given sequence along with the sequence specific data.

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