Plant Tissue Cult. 5(1) : 7-12, 1995

In vitro Rapid Shoot Proliferation and Corm Development in Gladiolus grandiflorus cv. Redbrand

Shelima Begum and Syed Hadiuzzaman

Department of Botany, University of Dhaka, Dhaka-1000, Bangladesh

Key words: Corm, Multiple shoots, Gladiolus, Proliferation

Abastract

A reproducible protocol for multiplication of Gladiolus Grandiflorus cv. Redbrand through axillary bud culture of corm has been established. The regeneration of multiple shoots is dependent upon the level and kind of cytokinin added to MS medium. Multiple shoot induction was best in half MS medium supplemented with 0.75 mg/1 BAP; healthy roots were produced in half MS supplemented with 0.5 mg/1 IBA. Corms were produced in vitro at the base of the shoots after complete differentiation of plantlets in the rooting medium.

Download Full Article