Plant Tissue Cult. 3(1) : 17-21, 1993

Callus Induction and Plant Regeneration from cultured Anthers of indica Rice Varieties

J.S. Sandhu, M.S. Gill* and S.S. Gosal

Biotechnology Centre, Punjab Agricultural University, Ludhiana 141004, India

Key words: Oryza sativa, indica rice, Anther culture, Callus induction, Plant regeneration

Abastract

Anthers containing pollen at late uninucleate stage from cold pretreated panicles at 4-5⁰C for 7 days of three varieties viz. Jaya, IR 54 and Vaigai were cultured on N₆ medium supplemented with various combinations and concentrations of auxins, cytokinins and sucrose. The best callusing from cultured anthers occurred on N₆ medium containing 2,4-D (1.75 mg/1), Kn (0.5 mg/1), sucrose (3% w/v) ranging from 1.75 per cent in Jaya to 2.25 per cent in IR 54. Upon transfer to N₆ medium supplemented with BAP (0.5 mg/1) and sucrose (4.5% w/v) anther-derived calli differentiated into shoots ranging from 15% in Jaya to 24% in IR 54.

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