Plant Tissue Cult. & Biotech. 18(2): 103-111, 2008 (December)

In vitro Clonal Propagtion of Trichosanthes cucumerina L. var. cucumerina

N. K. Devendra*, L. Rajanna, C. Sheetal and Y. N. Seetharam

Biosystematics and Medicinal Plant Laboratory, Department of Botany, Gulbarga University, Gulbarga-585 106, India

Key words: Trichosanthes cucumerina var. cucumerina, Clonal propagation, Shoot multiplication, Ontogeny

Abastract

An efficient protocol was established for in vitro shoot multiplication from shoot tip explant of Trichosanthes cucumerina L. var. cucumerina on semisolid MS basal medium supplemented with BA. NAA in the culture medium along with BA promoted higher number of shoot multiplication than BA alone. The rate of shoot multiplication was maximum 12.00 ? 0.70 after four weeks of culture on MS basal medium supplemented with BA 1.0 mg/l + NAA 0.1 mg/l. The elongated shoots rooted within seven - eight days in half strength of MS basal salts supplemented 1.0 mg/l IBA and 3% (w/v) sucrose. About 90% of the rooted plantlets were acclimatized and transferred to the greenhouse and successfully transferred to the field with 80% survival rate. The histological study shows that the organogenesis occurs directly, without callus formation on epidermal and sub epidermal layer of the explants. Adventitious shoots were characterized by the development of shoots apical meristem and leaf primordial.

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